静态顶空-气质联用分析淡豆豉中挥发性成分

Analysis of Volatile Components in Semen Sojae Praepatum with Automatic Static Headspace and Gas Chromatography-Mass Spectrometry

采用自动化静态顶空(HS)-气质联用(GC-MS)技术对6个批次淡豆豉的挥发性成分进行快速分析鉴定。从测定到的40多种成分中确定了2-丁酮、3-甲基丁醛、2-甲基丁醛、香芹烯等11种共有化合物及2,3,5-三甲基吡嗪、L-乙酸冰片酯、古巴烯、四甲基吡嗪等16种非共有化合物;同时使用峰面积归一化法计算了27种挥发性成分的相对含量,各组分的质量分数存在一定差异。研究表明,使用自动化静态顶空气质联用法测定淡豆豉的挥发性成分快速简便,且在一定程度上促进了淡豆豉挥发性成分的指纹图谱构建,为淡豆豉质量标准的建立提供了参考。

It was the first report on the volatile components contained in Semen Sojae Praepatum.For the analysis,the Semen Sojae Praepatum was analyzed by automatic static headspace and gas Chromatography mass Spectrometry.A total of 27 compounds were identified in Semen Sojae Praepatum through the computer retrieval on the NIST5 mass spectral library.They consisted of 11 generality components,such as 2-Butanone,Butanal 3-methyl-,Butanal 2-methyl-,Limonene and 16 special components,such as copaene,Pyrazine,tetramethyl-,2,3,5-Trimethyl-6-ethylpyrazine,Bicyclo[2.2.1]heptan-2-ol,1,7,7-trimethyl-,acetate,（1Sendo）-.Meanwhile,the quantitative analysis was taken using area normalization method,which showed that some difference was detected among six batches of Semen Sojae Praepatum.The results indicated that automatic static headspace and gas chromatography-mass spectrometry was a fast,easy,efficient and accurate method to analyze the volatile components in Semen Sojae Praepatum,and we thought that the findings may promote the fingerprint research of the volatile components in Semen Sojae Praepatum,to provide a scientific basis for the establishment of the quality standard.