摘要
利用荧光光谱法研究了月桂酸咪唑啉两性表面活性剂与牛血清白蛋白(BSA)的相互作用,用Stern-Volmer方程、Lineweaver-Burk方程确定了其荧光猝灭机理为静态猝灭,得到了在不同的温度和pH值条件下月桂酸咪唑啉两性表面活性剂和BSA作用的结合点位数、结合常数和热力学参数,研究结果表明其作用力主要为范德华力。
The interaction between lauric acid imidazoline and bovine serum albumin （BSA） is investigated by fluores- cence spectroscopy. It is proved that the fluorescence quenching mechanism belongs to the static quenching by Stern-Volmer equation and Lineweaver-Burk equation. Then the binding sites, binding constants and thermodynamics parameters of the interaction between lauric acid imidazoline and bovine serum albumin are obtained under the condition of different tempera- tures and pH values. The results show that Van der Waals＇ force plays a main role in the binding of lauric acid imidazoline and BSA
出处
《四川理工学院学报（自然科学版）》
CAS
2013年第4期18-21,共4页
Journal of Sichuan University of Science & Engineering(Natural Science Edition)
基金
四川省教育厅重点科研项目(10ZA031)
关键词
月桂酸咪唑啉
牛血清白蛋白
荧光光谱
结合位点数
结合常数
lauric acid imidazoline
bovine serum albumin
fluorescence spectroscopy
binding sites
binding constants
