探讨前S1抗原检测在乙肝检测中的作用

Application of pre-S1 antigen detection in hepatitis B diagnosing

目的探讨前S1抗原检测在乙肝检测中的作用。方法对患者血清前S1抗原、乙肝“两对半”和HBVDNA3项肝炎项目同时进行测定，并对结果进行回顾性分析。结果（1）在那些前S1抗原为阳性的标本中，HBsAg都检测出为阳性。在这些样本中，几种常见的乙肝“两对半”模式显示：“小三阳”模式阳性比较低，“15”模式阳性比例低，“大三阳”模式阳性比例高，“13”模式基本都为阳性。（2）HBVDNA检测为阳性的标本，前S1抗原检测仅有很少量的标本为阴性；HBVDNA检测为阴性的标本，有一定比例的前S1抗原检测为阳性。结论前S1抗原检测是对乙肝“两对半”，尤其是对HBVDNA测定的重要补充和加强，但可能存在较高的假阳性，据此目前认为HBVDNA检测仍然是评价病毒体内复制的金标准。

Objective To investigate the function of pre-S1 antigen testing in hepatitis B diagnosing. Methods Serum pre S1 antigen, serum markers of hepatitis B virus （HBV） and H13V DNA were detected among inpatients and outpatients of this hospital, and the results were retrospectively analyzed. Results （1） Among pre-S1 antigen positive samples, HBsAg were all positive, ＂tiny three positive＂ and ＂15＂ patterns possessed a low proportion, ＂ large three positive＂ pattern possessed a large proportion and ＂13＂ pattern were all positive. （2） Among HBV DNA positive samples, pre-S1 antigen were almost all positive except few samples. While among HBV DNA negative samples, pre-S1 antigen were positive in some samples. Conclusion Pre-S1 antigen testing could play an important role in further confirming the outcome of detection of serum markers of HBV and HBV DNA. However, the testing might suffer a proportion of false positive. Serum HBV DNA testing should still be the golden criterion in evaluating the virus duplicating in vivo.