免疫印迹法检测幽门螺杆菌抗体及其分型的结果分析

Assessment of experimental results of Helicobacter pylorri detection by immunoblotting

目的本文通过检测患者血清中幽门螺旋杆（Hp）抗体及分型，分析Hp抗体分型在临床上的感染情况，为临床诊断与治疗提供依据。方法随机选取该院门诊及住院患者476例，取空腹血用免疫印迹法检测Hp抗体谱，分析相应的Hp细胞毒素相关蛋白（CagA）、空泡毒素（VacA）、尿素酶（UreaseA、B）在不同人群中的表达情况。结果各年龄组对比年龄越大Hp感染率越高，疾病组对比胃炎，胃溃疡组Hp感染率最高，其他疾病，乙肝及体检患者感染率在40％～50％之间，差异无统计学意义。结论应用免疫印迹法可一次性检测Hp抗体谱，进行HP抗体分型有利于临床对上消化道疾病病情的判断和治疗；免疫印迹法检测幽门螺杆菌抗体具有较高的敏感性和特异性。方法简单，时间短，对临床治疗有重要指导意义。

Objective To analyze the characteristics of Helicobacter pylori （Hp） antibodies in patients to provide basis for clinical diagnosis and treatment. Methods 476 cases of outpatients and inpatients were randomly se lected and detected for Hp antibodies by Western blot testing and for Hp cytotoxin-associated protein （CagA）, vacuolating cytotoxin （VacA） and ureaseA and B. Results Infection rate of Hp increased with the increasing of age,those of gastritis and gastric ulcer groups were the highest, and those in hepatitis B patients and health examination subjects were 40% -50%, with no significant difference. Conclusion Hp antibodies detection by Western blot might be helpful for the judgment and treatment of diseases of upper digestive tract, with high sensitivity and specificity, be simple and low cost of detection time, which could have important significance for clinical treatment.