乳酸杆菌对过敏性鼻炎模型大鼠免疫功能的影响

Lactobacillus on allergic rhinitis rat models of the influence of the immune function

目的探讨乳酸杆菌对过敏性鼻炎(AR)大鼠血清中IL-4、IL-5、IFN-γ水平等免疫指标的影响。方法 SD大鼠60只随机分为正常对照组、模型组、乳酸杆菌组及阳性药对照组共4组,每组10只。乳酸杆菌组每天用乳酸杆菌活菌制剂灌胃3周;建立大鼠卵清蛋白致敏模型;阳性药对照组灌胃氯雷他定;另外20只大鼠用于被动皮肤过敏试验(PCA),检验大鼠模型制备及治疗情况;测定实验各组大鼠血清IL-4、IL-5、IFN-γ水平,检测鼻分泌物中嗜酸性粒细胞(EOS)水平。结果模型组IL-4、IL-5水平显著高于正常对照组(P<0.05),IFN-γ水平降低,低于正常对照组(P<0.05)。乳酸杆菌组IL-4、IL-5水平均显著低于模型组(P<0.05),IFN-γ水平增加,高于模型组(P<0.05)。乳酸杆菌组各项指标与阳性药对照组比较差异无统计学意义(P>0.05)。结论乳酸杆菌可下调AR大鼠血清中IL-4、IL-5水平,上调IFN-γ水平,调节Th1/Th2淋巴细胞亚群平衡,减轻鼻黏膜变应性炎症。

Objective Explore the effect lactobacillus on allergic rhinitis（AR）rat serum Il-4,Il-5,IFN-γconcentration and the influence of immune markers.Methods SD rat 60were randomly divided into 4groups,each group of 10only.Lactic acid bacteria group every day with lactobacillus living bacterium preparations irrigation stomach 3weeks,establish rats LuanBai protein sensitization model,positive drug control group irrigation stomach loratadine;Another 20rats used for passive cutaneous anaphylaxis（PCA）,inspection in the rat model preparation and treatment.Determination test group rats serum IL-4,IL-5,IFN-γ,the content of Eos in nasal secretion was detection.Results Model group IL-4,IL-5content was significantly higher than normal control group（P0.05）,IFN-γlevels were low,lower than the normal control group（P0.05）.Lactic acid bacteria group IL-4,IL-5content were significantly lower than the model group（P0.05）,IFN-γcontent increased,higher than the model group（P0.05）.The index of lactobacillus group with positive drug compared difference was statistically significant.Conclusion Lactic acid bacteria can cut AR rats serum IL-4,IL-5level,increase IFN-γlevel,adjust Th1/Th2lymphocyte subsets balance,reduce nasal mucosa allergic inflammation.