摘要
以带节茎段为外植体,研究福建野生葡萄的离体培养技术。结果表明:采用附加2.0mg.L-1 BA和0.2mg.L-1 NAA的MS固体培养基可诱导腋芽萌发,诱导率在72.2%;芽苗增殖时,综合考虑增殖系数和芽苗质量两方面因素,以附加2.0mg.L-1 BA和0.2mg.L-1 NAA的MS或1/2MS固体培养基的效果最佳,增殖系数可达3.0以上;作为离体种质保存时,以附加0.1mg.L-1 NAA的MS固体培养基为宜,继代间隔期可以达到5～6个月,并且保持芽苗的旺盛生长能力。
In vitro culture technique of Fujian wild grapes was studied by using the stem-segment with node as explants.The results showed that their axillary buds could be induced in MS medium supplemented with 2.0mg.L-1 BA and 0.2mg.L-1 NAA,in which the induction rate was 72.2%.Considering multiplication coefficient and plantlet quality comprehensively,the medium MS or 1/2MS added with 2.0mg.L-1 BA and 0.2mg.L-1 NAA was the best for the proliferation of fascicular gemma with the propagation coefficient over than 3.0.For in vitro germplasm conservation of Fujian wild grape plantlets,the suitable medium was MS with 0.1mg.L-1 NAA and the plastochrone of subculture were about 5to 6mouths.Consequently,the growth ability of plantlets could be maintained vigorously.
出处
《福建农业学报》
CAS
2013年第6期541-544,共4页
Fujian Journal of Agricultural Sciences
基金
福建省自然科学基金项目(2012J01102)
福建省财政专项--省属公益类科研院所基本科研专项(2011R1017-2)
福建省财政专项(闽财指[2011]724号)
福建省农业科学院博士科研启动基金项目(2010BS-3)
关键词
野生葡萄
离体培养
茎段
外植体
种质保存
wild grape
In vitro culture
stem-segment
explant
germplasm conservation
