摘要
[目的]研究新型光敏剂Ⅰ诱导Hep-2细胞的光氧化行为。[方法]利用MTT法检测光敏剂Ⅰ对Hep-2细胞的细胞毒性;采用活性氧特异性探针H2DCFDA通过激光共聚焦成像观察Hep-2细胞中活性氧的生成;通过测定超氧化物歧化酶(SOD)、谷胱甘肽(GSH)和丙二醛(MDA)水平及乳酸脱氢酶(LDH)渗漏检测观察Hep-2细胞的氧化应激反应。[结果]无光照时,光敏剂Ⅰ对Hep-2细胞的毒性为零,但光照后可明显抑制该细胞的生长,且其光毒性随光照剂量的增加而加强(r=-0.962,P=0.001)。光动力治疗后,细胞内DCFDA的荧光强度逐渐增强,在4h时达到高峰,随后又逐渐降低;细胞内SOD和GSH水平逐渐降低,3h后分别降低42.5%(P<0.01)和35.0%(P<0.01),而MDA含量却随时间延长逐渐增加,3h后增加54%(P<0.01)。LDH的渗出与光照剂量呈正相关(r=0.966,P=0.007)。[结论]新型光敏剂Ⅰ可有效光诱导Hep-2细胞死亡,而细胞内氧化应激反应可能是其光诱导Hep-2细胞死亡的重要作用机制。
[Purpose] To investigate the photooxidative action of Hep-2 cells induced by a novel photosensitizer Ⅰ. [Methods] The cytotoxicity of photosensitizer Ⅰ against Hep-2 cells was measured by MTT assay. The formation of active oxygen in the Hep-2 cells induced by photodynamic therapy(PDT) were observed by using an active oxygen specificity probe H 2 DCFDA through confocal laser scanning microscopy. The intracellular oxidative stress was investigated by determination of the levels of superoxide dismutase(SOD),glutathione (GSH) and malondialdehyde (MDA) in Hep-2 cells and the effusion of lactic dehydrogenase(LDH). [Results] No toxicity of photosensitizer Ⅰ on Hep-2 cells was observed when irradiation was not applied. Hep-2 cells were inhibited after PDT, and the photocytotoxicity of photosensitizer Ⅰ increased with the augmentation of irradiation(r=-0.962, P=0.001). After PDT,the fluorescence intensity of DCFDA in cells increased gradually,and reached the peak at 4h,then decreased gradually; the levels of SOD and GSH decreased gradually,with de- creased 42.5% (P0.01)and 35.0% (P0.01)respectively 3h after PDT; but the level of MDA in- creased with the prolongation of time,and increased 54% 3h after PDT (P0.01). The effusion of LDH was positively correlated with irradiation dose (r=0.966,P=0.007). [Conclusion] PDT mediated by photosensitizer Ⅰ can effectively induce the death of Hep-2 cells,and the oxidative stress in cells maybe the main mechanism.
出处
《肿瘤学杂志》
CAS
2013年第7期539-543,共5页
Journal of Chinese Oncology
基金
国家自然科学基金资助项目(21072227)