ATP生物荧光技术快速测定细菌总数的应用研究

RESEARCH ON FEASIBILITY OF ATP BIOLUMINESCENCE DETERMINATION OF THE TOTAL NUMBER OF BACTERIA

目的研究ATP生物荧光技术快速测定细菌总数的应用。方法通过生物荧光检测系统与其配套的试剂盒对细菌悬液进行定量检测,同时与平板活菌计数培养法作平行比较。结果经对金黄色葡萄球菌、大肠杆菌和绿脓杆菌菌悬液检测结果显示,三种细菌菌悬液检出菌数的对数值与ATP值的对数值之间呈线性关系。金黄色葡萄球菌、大肠杆菌和绿脓杆菌回归方程依次为Y=1.209+0.744X(R2=0.942),Y=0.885+0.831X(R2=0.961)和Y=0.955+0.953X(R2=0.983)。ATP生物荧光法适用于检测含菌量<107cfu/ml的菌悬液。结论 ATP生物荧光法可快速地评价受细菌污染的液体中细菌总数,但溶液中细菌含量应控制在<107cfu/ml。

Objective To research the feasibility of ATP bioluminescence assay rapidly determination of the total number of bacteria. Methods The kit biological fluorescence detection system and its supporting were used for quantitative detec- tion of bacterial suspension, and compare with the flat live bacteria counting culture method as parallel comparison. Results There was linear relationship between the logarithm of number of bacteria in Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa in suspensions and the logarithm of ATP （mol）value. Staphylococcus aureus regression equation was Y = 1. 209 ＋ 0. 744X （ R2 = 0. 942）, Escherichia coli regression equation was Y = 0. 885 ＋ 0.831X （ R2 = 0.961 ） ; and Pseudomonas aeruginosa regression equation was Y = 0.955 ＋ 0. 953X（ R2 = 0. 983 ）. It was suggested that ATP biolumi- nescence assay is suitable for the detection of bacteria in 〈 107 cfu/ml of bacteria suspension. Conclusions ATP biolu- minescence assay can be used to evaluate the total number of bacteria of contaminated liquid rapidly, but the solution of bacterial content should be controlled in 〈 107 efu/m].