DIP在肝细胞癌中的表达及其临床意义

DIP expression in hepatocellular carcinoma and its clinical significance

目的:探讨死亡诱导蛋白(DIP)在肝细胞癌(HCC)中的表达及其与肝癌临床病理特征之间的关系。方法:运用RT-PCR免疫组化分别检测DIP在40例HCC及其癌旁组织中的表达情况,并分析DIP蛋白表达与HCC临床病理特征之间的相关性;应用RT-PCR检测人正常肝细胞株LO2和HCC细胞Hep3B,HepG2,SMMC-7721中DIP mRNA的表达。结果:DIP mRNA及蛋白在肝癌组织中的表达明显高于其癌旁组织(均P<0.05);DIP蛋白高表达与较大肿瘤体积、高Edmonson分级和高TNM分期有关(r=0.419,0.414,0.531;均P<0.05);人HCC细胞株Hep3B,HepG2,SMMC-7721中DIP mRNA的表达均较正常肝细胞株LO2明显增高(均P<0.05)。结论:HCC组织中DIP表达上调,且这种表达上调与HCC的恶性病理特征相关。

Objective： To investigate the death inducing protein（DIP） expression in hepatocellular carcinoma（HCC） and its relations with the clinicopathological features of HCC. Methods： The mRNA and protein expressions of DIP in 40 HCC and their adjacent normal tissues were measured by RT-PCT and immunohistochemical staining respectively,and the relations of DIP protein expression with the clinicopathological features of HCC were analyzed.The DIP mRNA expressions in normal human liver cell line（LO2） and various human HCC cell lines（HepG2,Hep3B and SMMC-7721） were determined by RT-PCR method. Results： Both mRNA and protein expressions of DIP in HCC tissues were significantly higher than those in their adjacent tissues（both P〈0.05）,and high DIP expression was associated with larger tumor size,high Edmonson-Steiner＇s classification and advanced TNM stage（r=0.419,0.414,0.531;all P〈0.05）.The DIP mRNA expressions in all HCC cell lines（HepG2,Hep3B,and SMMC-7721） were significantly higher than that in LO2（all P〈0.05）. Conclusion： The DIP expression is up-regulated in HCC tissue,and the high DIP expression is associated with the unfavorable clinicopathological profiles of HCC.