MIF4GD在肝细胞肝癌中的表达及其对预后判断的价值

Expression of MIF4GD in hepatocellular carcinoma and its value in predicting prognosis

目的研究MIF4GD在肝细胞肝癌(HCC)中的表达及其对HCC预后判断的价值。方法 Western blot检测8例HCC及癌旁新鲜组织中MIF4GD表达。免疫组织化学法检测108例HCC及癌旁切片组织中MIF4GD及Ki-67表达。分析MIF4GD表达与HCC各临床病理参数间的相关性,并对病例进行随访,log-rank单因素和多因素Cox比例风险回归模型分析MIF4GD与HCC预后的关系。结果 MIF4GD在HCC组织中的表达明显低于相应的癌旁组织。MIF4GD的表达强度与HCC组织学分级、静脉侵袭和肝硬化状态相关(P<0.05),且MIF4GD表达与Ki-67呈负相关(r=-0.341,P<0.01)。MIF4GD低表达与HCC患者的不良预后显著相关(P<0.05)。结论MIF4GD在HCC中低表达,其可作为HCC独立的预后指标,提示MIF4GD可能与HCC的发生、发展密切相关。

Objective To investigate the expression of MIF4GD in hepatocellular carcinoma（HCC） and its value in predicting prognosis.Methods MIF4GD expression was determined by Western blot in 8 cases of fresh specimens of HCC and tumor-adjacent normal tissues.MIF4GD and Ki-67 expressions were detected by immunohistochemistry in 108 samples of HCC and tumor-adjacent normal tissues.Correlation between MIF4GD expression and clinicopathologic parameters of HCC was analyzed.The survival time of HCC patients with follow up was calculated with Kaplan-Meier analysis,and relationship between MIF4GD expression and prognosis of HCC was evaluated with univariate and multivariate Cox′s proportional hazards regression models.Results MIF4GD expression in HCC tissues was lower than that in tumor-adjacent normal tissues.MIF4GD expression was significantly correlated with histological grades,vein invasion and cirrhosis（P0.05）,but negatively correlated to Ki-67 expression（r=-0.341,P0.01）.Moreover,lower expression of MIF4GD was closely correlated with poorer prognosis（P0.01）.Conclusion MIF4GD expression is downregulated in HCC tissues and can be regarded as an independent prognostic indicator,which suggests that MIF4GD may play an important role in the oncogenesis and development of HCC.