冠心病患者调节性T细胞亚群的变化及其意义

Change and significance of regulatory T cell subsets in patients with coronary artery disease

目的探讨冠心病患者外周血调节性T细胞(Treg细胞)亚群的变化及其意义。方法将61例患者分为不稳定型心绞痛(UA组,21例)、稳定型心绞痛(SA组,18例)和胸痛综合征(CPS组,22例)三组。采用流式细胞仪分析CD4+CD25+Foxp3+Treg细胞占T淋巴细胞的比例;ELISA法检测血浆转化生长因子β1(TGF-β1)浓度。结果 UA组CD4+CD25+Foxp3+Treg细胞表达为(1.89±0.76)%,显著低于SA组的(2.91±0.53)%和CPS组的(2.35±0.26)%(P<0.01);UA组TGF-β1表达量为(13.52±3.02)μg/L,显著低于SA组的(26.94±7.25)μg/L和CPS组的(25.93±1.00)μg/L(P<0.01)。结论 UA组患者Treg细胞亚群比例降低,Treg细胞亚群与冠状动脉粥样硬化斑块的激活密切相关。

Objective To investigate the changes and significance of regulatory T cell subsets in the patients with coronary artery disease（CAD）.Methods Sixty-one patients were divided into 3 groups of UA（21 cases with unstable angina）,SA（18 cases with stable angina） and CPS（22 cases with chest pain syndrome）.The frequencies of CD4＋CD25＋Foxp3＋ regulatory T cell subsets in peripheral blood were detected by flow cytometry.Concentrations of transforming growth factor-β1（TGF-β1） were measured by ELISA.Results The percentage of CD4＋CD25＋Foxp3＋ regulatory T cell subsets in T lymphocytes in group UA was（1.89±0.76）%,which was significantly lower than（2.91±0.53）% in group SA and（2.35±0.26）% in group CPS（P0.01）.TGF-β1 expression in group UA was（13.52±3.02） μg/L,which was significantly lower than（26.94±7.25） μg/L in group SA and（25.93±1.00） μg/L in group CPS（P0.01）.Conclusion The regulatory T cell subsets are downregulated in the patients with UA.The regulatory T cell subsets are closely associated with the activation of vulnerable atherosclerosis plaque.