摘要
目的探讨Ad-hIFN-λ1重组腺病毒对胃腺癌细胞SGC-7901增殖的影响及其机制。方法分别用Ad-hIFN-λ1重组腺病毒、Ad-LacZ空质粒及PBS对照组作用于人胃腺癌细胞SGC-7901，MTT法测定细胞增殖情况，RT-PCR检测胃癌SGC-7901中IFN-λ1mRNA的表达，免疫荧光法检测hIFN-λ1蛋白的表达，Tunel、流式细胞术检测细胞凋亡。结果Ad-hIFN-λ1转染胃腺癌细胞SGC-7901后，肿瘤细胞生长明显抑制，细胞中hIFN-λ1 mRNA、蛋白高效表达，细胞凋亡率明显高于Ad-LacZ组、PBS空白对照组。结论Ad-hIFN-λ1重组腺病毒转染至胃腺癌细胞SGC-7901后表达IFN-λ1，Ad-hIFN-λ1能够显著抑制人胃腺癌SGC-7901细胞生长，并可能通过诱导其凋亡而起作用。
Objective To explore the effects of Ad-hIFN-λ1 recombinant adenovirus on prolifera- tion of gastric adenocareinoma cell line SGC-7901 and its mechanism. Methods Ad-hlFN-λ1 recombinant adenovirus and empty plasmid Ad-LacZ were respectively transfated to human gastric adenocarcinoma SGC- 7901 cells. The proliferation of transfected cells was detected by MTT assay. IFN-λ1l gene expression at mR- NA and protein levels in the ceils were measured by RT-PCR analysis and immunofluoreseenee assay, re- spectively. Tunel assay and flow eytometry were used to analyze the rate of cell apoptosis. Results The proliferation of gastric adenoeareinoma SGC-7901 cells were significantly inhibited with Ad-hlFN-λ1 inter- vention in accordance with highly expressed IFN-λ1at mRNA and protein levels, respectively. The apoptosis rate of Ad-hlFN-λ1 transfeeted cells was markedly higher than that of the empty plasmid Ad-LaeZ group and PBS blank control group. Conclusion The expression of hlFN-λ1 could be detected after transfection of Ad-hlFN-λ1 recombinant adenovirus into gastric adenocareinoma SGC-7901 cells. Ad-hlFN-λ1 could signifi- cantly inhibit the proliferation of gastric adenocarcinoma SGC-7901 cells by promoting the apoptosis of cancer cells.
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
2013年第7期507-512,共6页
Chinese Journal of Microbiology and Immunology
基金
江苏省镇江市2011社发科技支撑项目（SH2011037）
关键词
胃癌
IFN-λ
重组病毒
凋亡
Gastric cancer
IFN-λh
Recombinant adenovirus
Apoptosis
