三种呼吸道病毒实验室检测方法比较

A comparison of three methods for detecting respiratory viruses

目的在3种呼吸道多病原检测方法中筛选出敏感的实验室检测方法。方法以73份上呼道感染病例咽拭子标本作为检测对象，利用3种呼吸道多病原病毒检测方法，对17种呼吸道病毒检测指标进行平行检测，根据不同呼吸道病毒检测指标检出率，评价3种试剂检测效果。结果自建RT—PCR及PCR检测方法共检出56个阳性指标，市售多重RT-PCR检测试剂盒共检出41个阳性指标，市售多重实时荧光RT-PCR检测试剂盒共检出87个阳性指标。结论多重实时荧光RT—PCR检测试剂盒具有更高的检出率，可用于呼吸道多病原病毒的实验室检测。

Objective To screen a sensitive method for detecting respiratory viruses from three different methods of singleplex conventional PCR, multiplex conventional PCR and multiplex real-time RT- PCR. Methods Parallel examination of 17 respiratory viruses was performed on 73 throat swab specimens collected from patients with upper respiratory tract infection by the three methods. The detection rates of dif- ferent respiratory viruses were used as evaluating indicator for the three methods. Results The numbers of respiratory viruses detected by singleplex conventional PCR, muhiplex conventional PCR and multiplex real- time PCR were 56, 41 and 87, respectively. Conclusion The multiplex real-time RT-PCR might be used for the detection of respiratory viruses in laboratory as its high detection rate in comparison with the other two methods.