磺酸咔咯及其镓(Ⅲ)配合物与DNA的相互作用和光核酸酶活性

DNA Binding and Photonuclease Activity of Sulfonated Corrole and Its Gallium(Ⅲ) Complex

本文用紫外-可见光谱、荧光光谱、圆二色光谱和粘度法研究了2,17-二(磺酸钠基)-5,10,15-三(五氟苯基)咔咯(1)及其镓配合物(1-Ga)与小牛胸腺DNA(ct-DNA)的相互作用。结果表明1和1-Ga通过外部结合的方式与ct-DNA相互作用,且结合能力1-Ga比1大。琼脂糖凝胶电泳实验显示1和1-Ga均具较好的光核酸酶活性,1-Ga光断裂DNA效果比1好,其光断裂机理与羟基自由基的产生有关。

The interactions between 2, 17-bis（sodiumsulphonato）-5, 10, 15-tris（pentafluorophenyl） -corrole （1） and its gallium complex （1-Ga） with calf thymus DNA （ct-DNA） had been studied by UV-Vis absorption spectroscopy, fluorescence spectroscopy, circular dichroism spectroscopy and viscosity experiments. Results revealed that 1 and 1-Ga bind to ct-DNA via an outside（groove） binding mode and the DNA binding ability follows an order of 1- Ga〉1. Agarose gel electrophoresis experiments showed 1 and 1-Ga display good photonuclease activity with 1-Ga having a better DNA photocleavage performance and hydroxyl radical is the reactive species involved in the photocleavage process.