拟南芥叶形态建成基因ABL1的图位克隆及鉴定

Genetic Mapping and Identification of a Leaf Developmental Gene ABL1 in Arabidopsis thaliana

前期研究表明ABL1可能在植物叶发育过程中扮演重要的角色,其突变表现为叶片生长迟缓、成熟叶片叶缘缺刻明显等生长缺陷特征。该研究利用图位克隆及其精细定位技术,将ABL1基因锁定在2个SSLP标记T23K8和T8F5之间,该区间包含44个基因。通过生物信息学成功找到ABL1突变基因为拟南芥FAS1,该基因编码染色质组装因子CAF1的一个亚基,在植物顶端分生组织生长调控中扮演重要角色。RT-PCR结果显示,该基因表达受阻,功能互补实验证实abl1突变体的确是FAS1基因的一个新等位突变。研究结果暗示,ABL1/FAS1在植物叶形态建成中也起着重要作用。

Previous studies have shown that ABL1 may play an important role in the process of plant leaf growth and development,rosette leaves of the mutant abl1 formed later than wild-type and the mature leaves were smaller and serrate in abl1.In this study,using the Map-based cloning strategy,ABL1 gene was first positioning in the bottom region of the chromosome I by the primary mapping,and go a step further,ABL1 gene was limited in to the chromosomal region between two molecular markers T23K8 and T8F5,which contains 44 genes.A mutation in genes FAS1 was responsible for the mutant phenotype by bioinformatics analysis.FAS1 encode a subunit of the chromatin assembly factor CAF1,which regulate the apical meristem of the plant.RT-PCR results have indicated that the expression of ABL1 gene is completely knocked out and complementation experiments confirmed that abl1 is indeed a new allelic mutation in gene FAS1.Based on the study above,ABL1 may play a crucial role in the plant leaf morphogenesis.