紫苏内生真菌Aspergillus sp.12Y03化学成分研究

Chemical Constituents from an Endophytic Fungus Aspergillus sp.12Y03 Isolated from Perilla frutescens

采用硅胶柱层析和Sephadex LH-20凝胶柱层析等分离方法,从紫苏内生真菌Aspergillus sp.12Y03发酵产物中分离鉴定了10个化合物,经现代光谱学技术鉴定为:环-(脯氨酸-甘氨酸)(1)、环-(脯氨酸-丝氨酸)(2)、环-(丝氨酸-4-OH-脯氨酸)(3)、环-(丙氨酸-4-OH-脯氨酸)(4)、环-(苯丙氨酸-甘氨酸)(5)、环-(丙氨酸-甘氨酸)(6)、亚油酸(7)、α-亚麻酸(8)、cerevisterol(9)和22E,24R-5α,6α-环氧麦角甾-8(14),22-二烯-3β,7α-二醇(10),均为首次从该菌种中分离得到,化合物3和4具有中等强度的海虾致死活性。

Ten compounds were isolated from the culture of an endophytic fungus Aspergillus sp.12Y03 in Perilla frutescens.Their structures were elucidated by spectroscopic methods as cyclo-（Pro-Gly）（1）,cyclo-（Ser-Pro）（2）,cyclo-（Ser-4-OH-Pro）（3）,cyclo-（Ala-4-OH-Pro）（4）,cyclo-（Gly-Phe）（5）,cyclo-（Gly-Ala）（6）,linoleic acid（7）,α-linolenic acid（8）,cerevisterol（9） and 22E,24R-5α,6α-epoxyergosta-8（14）,22-diene-3β,7α-diol（10）.All compounds were isolated from this fungus for the first time.Compound 3 and 4 was shown to be moderate active toward brine shrimp larvae.