糖尿病与正常大鼠骨髓间充质干细胞移植治疗心肌梗死的比较

Bone marrow mesenchymal stem cells from diabetic versus normal rats for treatment of myocardial infarction

背景: 目前国内外在干细胞移植治疗心肌梗死的研究中,大多采用正常或者年轻的骨髓间充质干细胞作为移植细胞来源。目的: 比较糖尿病和正常大鼠骨髓间充质干细胞移植治疗心肌梗死的疗效差别。方法: 无菌条件下获取正常大鼠及糖尿病大鼠的骨髓间充质干细胞,建立大鼠心肌梗死模型并随机分为3组,分别于心肌梗死病灶区注射100μL含有105-106个F2代正常大鼠或糖尿病大鼠来源骨髓间充质干细胞的细胞混悬液,空白对照组注射100μL含体积分数20%胎牛血清的DMEM。移植后1个月行苏木精-伊红染色检测各组梗死区域心肌组织形态的变化;通过免疫组化方法检测Bcl-2的表达。结果与结论: 通过对细胞形态观察及流式细胞术鉴定,大鼠股骨骨髓贴壁培养可获取纯度较高的骨髓间充质干细胞,绘制细胞生长曲线结果显示正常大鼠骨髓间充质干细胞较糖尿病大鼠骨髓间充质干细胞生长快。移植后1个月,正常大鼠干细胞移植组梗死区域心肌组织形态较糖尿病大鼠干细胞移植组及空白对照组明显改善,梗死区域心肌组织Bcl-2的表达也高于其他2组。证实正常大鼠来源骨髓间充质干细胞较糖尿病大鼠骨髓间充质干细胞生长明显加快,且糖尿病使骨髓干细胞移植治疗心肌梗死的疗效降低。

OBJECTIVE： To compare the therapeutic effects of bone marrow mesenchymal stem cells from diabetic and normal rats on myocardial infarction. METHODS： Under sterile conditions, bone marrow mesenchymal stem cells from normal and diabetic rats were harvested. Then, rat models of myocardial infarction were established and randomly divided into three groups： 100 IJL cell suspension containing 105-106 bone marrow mesenchymal stem cells from F2 normal or diabetic rats was injected into myocardial infarction lesions, and 100 pL Dulbecco＇s modified Eagle＇s medium containing 20% fetal bovine serum was injected serving as blank control. After I month, hematoxylin-eosin staining for myocardial infarction lesions was performed for histomorphological observation. Bcl-2 expression was detected by immunohistochemistry method. RESULTS AND CONCLUSION： Based on ceil morphology observation and flow cytometry identification, high-p0rity bone marrow mesenchymal stem cells could be obtained using rat femoral bone marrow adherent culture. Cell growth curve showed that normal rat bone marrow mesenchymal stem cells grew faster than those from diabetic rats. At 1 month after transplantation, histomorphological improvement was seen in the infarctedarea after transplantation of normal rat bone marrow mesenchymal stem cells as compared with the other two groups. In addition, the Bcl-2 expression in the infarcted area was higher in the normal rat cell group than the the other two groups. These findings indicate that bone marrow mesenchymal stem cells from normal rats grow faster than those from diabetic rats, and the cells from normal rats have better therapeutic effects on myocardial infarction,