宫颈癌组织中凋亡因子表达的分析

Expression of apoptotic factors in the tissues of cervical cancer

目的:探讨凋亡因子表达水平与宫颈癌发生、发展过程的关系。方法:免疫组化法检测宫颈癌组织标本中NF-κB、Caspase-3及Bcl-2表达。结果:NF-κB及Caspase-3在宫颈浸润性鳞细胞癌中表达阳性率较宫颈原位鳞细胞癌显著降低(P<0.01),且二者表达呈对应关系;Bcl-2的表达均为阴性。结论:NF-κB与Caspase-3相互拮抗调节宫颈鳞细胞癌的发展,这种调控的变化可能与肿瘤的恶性转化相关,调控NF-κB与Caspase-3的拮抗表达值得作为宫颈鳞细胞癌治疗的一个新研究方向。Bcl-2表达阴性提示在宫颈鳞细胞癌发生、发展中,线粒体凋亡通路可能缺失。

Objective：To investigate the relationship between the expression level of apoptotic factors and the occurrence and development of cervical cancer.Methods： Immunohistochemical stain was used to detect the expression of NFκ-B,Caspase-3 and Bcl-2 in tissue samples from the patients with cervical squamous cell cancer.Results： The positive rates of expressions of NFκ-B and Caspase-3 were significantly reduced in the tissues of invasive cervical squamous cell carcinoma than that of in situ cervical squamous cell carcinoma,and their expression were corresponding（P0.01）.No expression of Bcl-2 was found.Conclusion： The antagonism between Caspase-3 and NFκ-B regulates the development of cervical squamous cell carcinoma,and the changes of Caspase-3 and NFκ-B expression level are related with malignant transformation.The antagonistic regulation of Caspase-3 and NFκ-B is a new research direction in cervical squamous cell carcinoma.The negative expression of Bcl-2 implied that mitochondrial apoptotic pathway may be lost in the development of cervical squamous cell carcinoma.