多发性硬化患者外周血调节性T细胞功能受损

Functional defect of regulatory T cells in peripheral blood of patients with multiple sclerosis

目的:探讨多发性硬化患者外周血调节性T细胞(Treg)的变化及其转录因子Foxp3的表达。方法:收集58例多发性硬化(MS)患者及24例正常人外周血,通过流式细胞术检测CD4+CD25+T细胞,RT-PCR检测Foxp3的表达。结果:MS患者外周血中Treg细胞数量较正常人无差异(P>0.05),而Foxp3表达明显减低(P<0.05)。结论:MS患者外周血中Treg细胞数量较正常人无明显变化,但其功能明显受损,是导致自身免疫性疾病发生的原因之一。

Objective：To detect regulatory T cells and Foxp3 expression in peripheral blood of patients with multiple sclerosis（MS）.Methods： A total of 58 MS patients and 24 healthy people were included in this study,the number of CD4＋CD25＋ regulatory T cells in peripheral blood were detected by flow cytometry,Foxp3 expression were analyzed by real-time fluorescent quantitative RT-PCR.Results： There was no difference of the number of Treg cell between MS patients and healthy people,but the expression of Foxp3 were significantly decreased in MS patients（P0.01）.Conclusion： The function of Treg cells were severely impaired in MS patients although its number had not changed compared with healthy people,which could contribute to the onset of this autoimmune disease.