CCR2-V64I基因单核苷酸多态性与宫颈癌及宫颈上皮内瘤变的相关性研究

Correlation of CCR2-V64I gene single-nucleotide polymorphism with cervical cancer and cervical intraepithelial neoplasia

目的探讨趋化因子受体-2(CCR2)基因的单核苷酸多态性(SNP)位点V64I(CCR2-V64I)与宫颈癌及宫颈上皮内瘤变(CIN)的相关性。方法采用聚合酶链式反应-序列特异性引物(PCR-SSP)、琼脂糖凝胶电泳及基因测序联合检测分析方法,对40例原发性宫颈癌、45例CIN和60例同期健康体检者进行基因多态性的检测。结果基因测序与琼脂糖凝胶电泳对CCR2-V64I的检测结果一致。CCR2-V64I G/G、G/A和A/A基因型频率在宫颈癌中分别为27.5%、12.5%和60.0%,在CIN中分别为53.3%、24.4%和22.2%,而在对照组中分别为38.3%、31.7%和42.9%,宫颈癌组与对照组的基因型分布差异有统计学意义(P<0.01),但CIN组与对照组之间差异无统计学意义(P>0.05)。携带A等位基因可以增加宫颈癌的发病风险(OR=2.32,95%CI:1.29～4.17),但并不增加CIN的发病风险(OR=0.62,95%CI:0.35～1.09)。结论宫颈癌及CIN中均存在CCR2-V64I基因多态性,但其与CIN无关,而与宫颈癌患病风险有关,表明CCR2-V64I基因多态性在宫颈癌的发生、发展中可能具有重要意义。

Objective To investigate the relationship between the gene single-nucleotide polymorphisms（SNP） site of V64I within chemokine receptor 2 （CCR-2） and cervical cancer and cervical intraepithelial neoplasia（CIN）. Methods Polymerase chain reaction sequence specific primers （PCR-SSP）, electrophoresis and gene sequencing analysis were applied respectively to detect the CCR2-V64I gene SNP in 40 patients with primary cervical cancer,45 patients with CIN and 60 health controls. Results Gene sequen- cing analysis and electrophoresis showed the same results on the CCR2-V64I gene SNP. The frequencies of G/G, G/A and A/A geno- type of V64I polymorphism within CCR2 gene were 27.5% ,12. 5% and 60.0% in cervical cancer, 53.3%, 24.4% and 22. 2% in CIN, and 38. 3%, 31.7% and 42. 9% in control group. There was significant different in the distribution of polymorphism of CCR2- V641 gene polymorphism between cervical cancer and normal control（P 〈0.01）, but there was no significant difference between CIN and the normal control（ P 〉 O. 05 ）. Harboring allele A increased the risk of cervical cancer （ OR = 2. 32,95% CI ： 1.29-4. 17 ）, but did＇t increase the risk of CIN（OR =0.62,95% CI：0. 35-1.09）. Conclusion CCR2 gene polymorphism exists in cervical cancer and CIN, which correlates with the risk of cervical cancer but not with CIN, indicating that CCR2-V641 gene polymorphism may play an important role in the occurrence and development of cervical cancer.