Cyclin D1、Ki-67及PTEN在乳腺癌中的表达及意义

Expression and clinical significance of Cyclin D1,Ki-67 and PTEN in breast cancer and breast hyperplasia

目的:研究细胞周期蛋白D1(Cyclin D1)、Ki-67蛋白及第10号染色体同源缺失磷酸酶-张力蛋白基因(phosphatase and tensin homologue deleted on chromosome ten,PTEN)在乳腺癌组织中的表达,并探讨其在乳腺癌、乳腺增生症中的意义。方法:运用免疫组织化学SP法,对120例乳腺浸润性导管癌(A组)及20例乳腺增生症(B组)标本进行Cyclin D1、Ki-67及PTEN检测。结果:Cyclin D1在乳腺浸润性导管癌(A组)中的阳性表达率为59.2%(71/120),在乳腺增生症(B组)的阳性率为15.0%(3/20),差别具有统计学意义(P<0.05)。Ki-67在A组中的阳性率为80.0%(96/120),在B组的阳性率为10.0%(2/20),差别具有统计学意义(P<0.05)。PTEN在A组中的阳性率为49.2%(59/120),在B组的阳性率为90.0%(18/20),差别具有统计学意义(P<0.05)。结论:在乳腺浸润性导管癌中,Cyclin D1和Ki-67明显高于乳腺增生症,PTEN阳性表达低于乳腺增生症,并且三者之间存在关联性,可以作为乳腺癌预后和靶向治疗指标应用于临床。

Objective：To study the expressions of cyclin D1,Ki-67 protein and phosphatase and tensin homologue deleted on chromosome ten（PTEN） in breast invasive ductal carcinoma,and to explore its clinical significance in breast invasive ductal carcinoma.Methods：The expression of Cyclin D1,Ki-67 and PTEN was evaluated in 120 cases of breast invasive ductal carcinoma（group A） and 20 cases of breast hyperplasia（group B） by immunohistochemistry SP method.Results：The positive expression rates of Cyclin D1 in breast invasive ductal carcinoma（group A） was 59.2%（71 /120）,but in breast hyperplasia（group B）,the positive rates was 15.0%（3 /20）,the difference was statistically significance（P 0.05）.The positive rates of Ki-67 in group A 80.0%（96 /120）.For group B,the positive rates of 10.0%（2 /20）,and the difference was statistically significant（P 0.05）.The positive rates of PTEN were 49.2%（5 9/1 2 0） and 9 0.0 %（1 8/2 0） in group A and group B respectively.The difference was significant（P 0.05）.Conclusion：In breast invasive ductal carcinoma,the positive expression rates of Cyclin D1 and Ki-67 significantly higher than breast hyperplasia,the positive expression rates of PTEN are significantly lower than breast hyper plasia.Therefore,clinically,these factors could be used as indicators for breast cancer prognosis and targeted therapy.