不同长度兔桡骨缺损区骨形态发生蛋白2的表达

Bone morphogenetic protein 2 expression in rabbit radial defect site with different lengths

背景:有研究表明在骨缺损的状态下,骨形态发生蛋白2的分布存在一定的规律。目的:观察不同长度兔桡骨骨缺损部位骨形态发生蛋白2的表达。方法:将48只新西兰大白兔随机分成2组,分别在麻醉下用线锯造成兔左前臂桡骨中段0.5和3.0cm的骨缺损。结果与结论:Western blot检测显示,0.5cm骨缺损组骨缺损部位骨形态发生蛋白2蛋白表达量在损伤后1,3,4周呈逐渐增多的趋势,且每组均较损伤即刻明显增加(P<0.05);3.0cm骨缺损组骨缺损部位的骨形态发生蛋白2的相对表达量在损伤后3周达到高峰(P<0.05),其峰值水平明显高于0.5cm骨缺损组(P<0.05),基本维持在较高水平。对骨痂的生成情况进行量化评估结果显示,损伤后3,4周,3.0cm骨缺损组的骨痂生成量较0.5cm骨缺损组明显减少(P<0.05)。证实,兔桡骨3.0cm的骨缺损部位骨形态发生蛋白2表达明显上调,但还不能使该长度的骨缺损自行愈合。

BACKGROUND：It has been studied that the distribution of bone morphogenetic protein 2 is regular under bone defect situation. OBJECTIVE：To observe the expression of bone morphogenetic protein 2 in rabbit radial defect site with different lengths. METHODS：Forty-eight New Zealand rabbits were divided into two groups randomly, 0.5 cm bone defect and 3.0 cm bone defect were made by wire saw at the middle part of radius bone after anaesthesia. RESULTS AND CONCLUSION：Western blot results showed that in the 0.5 cm bone defect group, the expression of bone morphogenetic protein 2 of the tissues in the bone defect site was increased gradual y at 1, 3, 4 weeks after operation, and the expression in each defect group was increased when compared with that immediately after injury （P〈0.05）. In the 3.0 cm bone defect group, the expression of bone morphogenetic protein 2 of tissues in bone defect site was increased gradual y and reached to its peak at 3 weeks after the operation （P〈0.05）, and the peak value in the 3.0 cm bone defect group was significantly higher than that in 0.5 cm bone defect group （P〈0.05）. The peak value was maintained in high level. The comparison of bone cal us formation showed that the bone cal us formation of 3.0 cm bone defect group was less than that of the 0.5 cm bone defect group at 3 and 4 weeks after operation （P〈0.05）. The results indicate that expression of the bone morphogenetic protein 2 in 3.0 cm bone defect site is increased significantly, but the expression level cannot make the bone defect heal itself.