三七总皂苷对腹主动脉缩窄致大鼠心肌肥厚能量代谢紊乱的抑制作用

Inhibition Effect of Panax Notoginseng Saponin on Cardiac Hypertrophy and Energy Metabolism Disorder Induced by Abdominal Aorta Constriction in Rats

目的探讨三七总皂苷(PNS)对腹主动脉缩窄致大鼠心肌肥厚的抑制作用及机制。方法取75只大鼠,采用腹主动脉缩窄法致心肌肥厚;随机取15只大鼠进行假手术作为对照(假手术组)。1周后,手术大鼠随机分为4组:模型组、低剂量PNS组(50 mg/kg)、中剂量PNS组(100 mg/kg)和高剂量PNS组(150 mg/kg)。给药11周,测定大鼠血流动力学改变;计算心脏指数和左心室质量指数;病理切片作HE染色观察大鼠左心室心肌形态学改变;取出左心室部分心肌组织进行乳酸、游离脂肪酸测定;采用逆转录聚合酶链反应测定心肌组织心房钠尿肽mRNA的表达;采用高效液相色谱法测定心肌中的三磷酸腺苷(ATP)、二磷酸腺苷(ADP)和一磷酸腺苷(AMP)含量。结果与模型组相比,PNS可以降低肥厚指数,改善其血流动力学,降低心房钠尿肽mRNA的表达,降低心肌肥厚大鼠心肌乳酸和游离脂肪酸含量,增高心肌ATP、ADP和AMP含量。结论 PNS能够有效抑制腹主动脉缩窄大鼠心肌肥厚并改善其能量代谢紊乱。

Aim To explore effects and mechanism of panax notoginseng saponin （PNS） on hypertrophic myocardium caused by abdominal aorta constriction（AAC） in rats. Methods Rat left ventricular hypertrophy was induced by abdominal aorta constriction in 75 rats, and 15 rats were randomly taken as sham group. One week after surgery, rats were divided into 4 groups： model group （abdominal aorta constriction rats）, low-dose PNS group （50 mg/kg）, middle-dose PNS group （100 mg/kg） and high-dose PNS group （150 mg/kg）. After accepting therapy for 11 weeks, the hemodynamics of all animals was detected the left ventricle was recorded to calculate left ventricular hypertrophic parameters including left ventricular hypertrophy index （HMI, the ratio of the heart and body weight and LVMI, the ratio of the left ventricular weight and body weight） pathological section was stained by hematoxylin-eosin（HE） the lactic acid （LAC） and free fatty acids（FFA） were measured the mRNA expression of heart atrial natriuretic peptide （ANP） was observed through reverse transcription-polymerase chain reaction （RT-RCR） the contents of adenosine triphosphate （ATP）, adenosine diphosphate（ADP） and adenosine monophosphate （AMP） in rat were detected by high performance liquid chromatography （HPLC）. Results Compared with the AAC model group, PNS was able to inhibit cardiac hypertrophy, ameliorate hemodynamics, the expression of atrial natriuretic peptide was decreased, the lactic acid （LAC） and free fatty acid （FFA） levels was decreased, and the content of ATP, ADP and AMP was largely increased. Conclusions PNS protection against left ventricular hypertrophy elicited by abdominal aorta constriction in rats is mediated, at least in part, via ameliorate energy metabolism.