塞来昔布联合三苯氧胺对甲基亚硝基脲诱导大鼠乳腺肿瘤的影响

Celecoxib combined with tamoxifen prevents methylnitrosourea-induced breast tumor in rats

目的:塞来昔布联合三苯氧胺对甲基亚硝基脲(MNU)诱发大鼠乳腺肿瘤的影响。方法:雌性3周龄SPF级SpragueDawley(SD)大鼠140只随机分成对照组、塞来昔布组、三苯氧胺组及联合组,腹腔注射MNU后分别给予不同的方案进行干预,观察各组大鼠乳腺肿瘤的发生率、肿瘤的体积及COX-2和C-erbB-2的表达。结果:塞来昔布组、三苯氧胺组与对照组比较,肿瘤发生率低,肿瘤体积小;联合组肿瘤发生率最低,肿瘤体积最小。塞来昔布组、联合组大鼠乳腺肿瘤COX-2、C-erbB-2的阳性表达率低于对照组和三苯氧胺组(P<0.05)。结论:塞来昔布和三苯氧胺对MNU诱发大鼠乳腺肿瘤的发生均有预防抑制作用,两药联合效果更好。

Objective： The present study aims to evaluate the chemopreventive effect of celecoxib combined with tamoxifen on breast tumor induced by methylnitrosourea （MNU） in rats. Methods： A total of 140 SD female rats were injected with MNU to estab- lish breast tumor models. The rats were divided subsequently into control, celecoxib, tamoxifen, and combination groups. The occur- rence rates, volumes of breast tumor, and expression levels of cyclo0xygenase 2 （COX-2） and c-erbB-2 were observed. Results： The tu- mor occurrence rates were lower in the celecoxib and tamoxifen groups than in the control group. The combination group exhibited the lowest tumor-occurrence rate. The tumor volumes of the celecoxib and tamoxifen groups were lower than that of the control group. The combination group had the least tumor volume. The positive rates of COX-2 and c-erbB-2 in the celecoxib and combination groups were lower than those in the control and tamoxifen groups （P〈0.05）. Conclusion： The combination of celecoxib and tamoxifen can sig- nificantly suppress MNU-induced breast tumor in female rats.