Oct-4蛋白在直肠癌中的表达及其与患者预后的相关性

Expression of Oct-4 protein in rectal adenocarcinoma and its correlation with prognosis

目的观察直肠腺癌组织中胚胎干细胞标志物Oct-4蛋白的表达,并对其与直肠癌患者预后的关系进行探讨。方法应用免疫组织化学ABC法检测52例直肠癌组织中Oct-4蛋白的表达,分析Oct-4蛋白表达与预后、分期、分化程度的关系。结果 Oct-4蛋白表达主要位于直肠癌细胞胞质内,其阳性细胞数为0%～18.5%,表达阴性和阳性者分别有38例(73.1%)和14例(26.9%)。Oct-4表达阳性率与性别、年龄、临床分期和病理学分级之间无相关性(P>0.05)。Oct-4表达阴性和阳性者的3年局部无复发率分别为83.5%和75.0%,两者间差异无统计学意义(P=0.583);3年无远处转移率分别为88.6%和61.9%,两者间差异有统计学意义(P=0.035);3年生存率分别为77.9%和50.0%,差异有统计学意义(P=0.037)。结论 Oct-4在直肠腺癌组织中有不同程度的表达,Oct-4阳性表达与远处转移率及总生存率相关,与3年局部无复发率无关。

Objective To investigate the expression of embryonic stem cell marker Oct-4 protein in rectal adenocarcinom and its correlation with prognosis. Methods The expression of Oct-4 protein was evaluated by immunohistochemisti7 in 52 formalin-fixed paraffin-embedded tissue samples of rectal adenocarcinoma. The correlation of Oct-4 expression with clinical outcome was analyzed by Kaplan-Meier and log-rank methods. Results The positive expression rate of Oct-4 protein in rectal adenocarcinoma was 26.9% （ 14/ 52）, which was not correlated with gender, age, clinical stage, and histological grade（P 〉 0.05 ）. The 3-y local recurrence-free rates in patients with negative and positive Oct-4 expression were 83.5% and 75.0% , respectively （ P = 0. 583 ） , the 3-y metastasis-free rates were 88.6% and 61.9% , respectively （P = 0. 035 ） and the 3-y overall survival rates were 77.9% and 49.0% , respectively （ P = 0. 037 ）. Conclusion Embryonic stem cell marker Oct-4 expression may have prognostic significance in patients with rectal adenoearcinoma