摘要
从土壤中筛选培育出一株脲酶高产菌株,经16S rDNA鉴定为迟缓芽孢杆菌(Bacillus lentus)。利用该菌株制备微生物砂浆,所制备的砂浆单轴抗压强度为2.4MPa。对所产生的物质进行X射线衍射分析,结果表明所形成的物质为方解石和球霰石,每克砂柱中碳酸钙的含量为163.15mg/g。通过扫描电子显微镜观察发现砂粒之间有球状及六面体状碳酸钙结晶生成,粘结后的砂柱中仍存在大量孔隙。
A high yield urease produced strain is isolated from soil, It is identified as Bacillus lentus by 16S rDNA sequences. Mortar is prepared using the strain, uniaxial compressive strength of it is 2.4 MPa. Analysis by XRD, the new material is calcite and vaterite. The yield of calcium carbonate in sand column is of 163.15 mg/g sand. Through SEM ob- servation found hexahedral shaped calcium carbonate crystallization generated, and there are still a large number of pores in the sand column after bonding.
出处
《混凝土与水泥制品》
北大核心
2013年第8期13-16,共4页
China Concrete and Cement Products
基金
国家自然科学基金(50908122)
关键词
迟缓芽孢杆菌
微生物矿化
碳酸钙
脲酶
Bacillus lentus
Microbial mineralization
Calcium carbonate
Urease
