山奈黄素光度法测定烟草样品中的铝

Spectrophotometric Determination of Aluminum(Ⅲ) in Tobacco with Kaempferol

本文研究了山奈黄素与铝的显色反应 ,在 p H=5 .6 HAc- Na Ac缓冲介质中 ,有 CPB存在下 ,山奈黄素与铝反应生成 3∶ 1稳定络合物 ,λmax=42 5 nm,ε=1 .6 3× 1 0 4 L· mol- 1 · cm- 1 。铝含量在 0— 2 0μg/2 5 m L内符合比耳定律 ,方法用于烟草样品中铝含量的测定 ,结果令人满意。

The color reaction of kaempferol with aluminum(Ⅲ) was studied.In the presence of pH 5.6 buffer solution and CPB medium,kaempferol can react with aluminum(Ⅲ) to form a stable 3∶1 complex.The malor absorptivity is 1.63×10 4L·mol -1 ·cm -1 at 425nm.Beer's law is obeyed in the range of 0—20μg/25mL.This method can be applied to the determination of aluminum in tobacco with satisfactory results.