新亚铜灵试剂光度法测定烟草中的铜

Study on Spectrophotometric Determination of Copper in Tobacco with Neocuproin Hydrochlorid Monohydrate

研究了在 Emulsifier- OP存在下新亚铜灵试剂 (NHCM)与铜的显色反应 ,在 p H为 4.5的 HAc- Na Ac缓冲介质中 ,Emulsifier- OP存在下 ,NHCM与铜反应生成 2∶ 1稳定络合物 ,λmax=46 0 nm,ε=1 .6 4× 1 0 4 L·mol- 1 ·cm- 1 。铜含量在 0— 5 0 μg/2 5 m L 范围内符合比耳定律 ,该方法用于烟草中铜含量的测定 ,结果令人满意。

The color reaction of copper with neocuproinhydrochlorid monohydrate(NHCM) was studied.In pH 4.5 HAc NaAc buffer solution and emulsifier OP medium,NHCM can react with copper(Ⅱ) to form a stable 2∶1 complex.The molor absorptivity is 1.64×10 4L·mol -1 ·cm -1 at 460nm.Beer's law is obeyed in range of 0—50μg/25mL.This method can be applied to the determination of copper in tobacco with satisfactory results.