吖啶橙(AO)-藏红T(ST)荧光探针测定DNA的研究

STUDY ON THE DETERMINATION OF DNA USING ACRIDINE ORANGE SAFRANINE T AS FLUORESCENCE PROBE

简述了以荧光能量转移体系测定 DNA方法的发展过程 ,同时对吖啶 (AO) -藏红 T(ST)荧光探针测定 DNA的条件进行分析 ,证明了该法灵敏度高、线性范围宽、检出限低、选择性好、抗干扰能力强 ,该法为微量

A brief review on the recent progress of the determination of DNA using the fluorescence energy transfer system, and a thorough study on the optimum conditions for the determination of DNA using acridine orange safranine T reaction system as fluorescence probe is given in this paper. It is proved that the above mentioned method has the predominant advantages of high sensitivity and selectivity, low detection limit and wide range of linearity, and that it is a new and preferable approach for the determination of DNA.