油棕基因组DNA 3种提取方法的比较研究

Comparison of Three Extract Methods for Genomic DNA of Elaeis guineensis

采用CTAB小样提取法、PVP法和SDS-CTAB改良法,分别从油棕嫩叶和老叶中提取基因组DNA,并通过紫外分光光度计、琼脂糖凝胶电泳和PCR反应进行检测。研究结果表明:用CTAB小样提取法提取的油棕嫩叶总DNA的质量最好,纯度最高;用其他2种方法提取的DNA的质量均较差。

Using CTAB extraction with small sample, PVP method and SDS - CTAB improved method respectively, Genomic DNAs were extracted from the young and mature leaves of Elaeis guineensis Jacq. , and were tested by the ultraviolet speetrophotome- ter, agarose gel electrophoresis and PCR reaction. The results showed the extracted total DNA from the young leaves of Elaeis guineensis had the best quality and the highest purity by the CTAB extraction with small sample, but the extracted DNA by other two methods was of ooor aualitv.