铂黑修饰酶电极测定胆碱

Quantitative Determination of Choline with Glassy Carbon Electrode Mourned with Platinum-black and Choline Oxidase

利用电化学方法在玻碳电极上均匀沉积亚微米粒度铂黑并进而修饰一层胆碱氧化酶膜，制成了胆碱酶电极。以计时电流法测定氯化胆碱，检测下限为 ７．４ μｍｏｌ／Ｌ，线性范围２．４×１０－２～ １．０ ｍｍｏｌ／Ｌ，重现性好，使用寿命较长。

An enzyme for choline was fabricated by modifying an enzyme layer composing of choline oxidase on the surface of sub-micrometer platinum-black particles,which was electro-deposited on the surface of glassy carbon electrode. Chronoamperometry was applied for quantitative determination of choline. Experimental results indicated this enzyme electrode gave a wide linear detection from 2.4 ×10-2 to 1.0 mmol/L of choline and low detection limit of 7.4 μmol/L. The enzyme electrode was also reproducible and good for long-term use.