化学计量学法辅助紫外分光光度法同时测定小儿复方苯巴比妥片剂中四组分的研究

Studies on Simultaneous Determination of Four Components in Compound Child's Phenobarbital Tablet Using UV Spectrophotometry Assisted by Chemometrics Methods

在质量浓度为 0 .5g/L的酒石酸乙醇溶液中 ,阿司匹林、非那西汀、咖啡因和苯巴比妥的 λmax分别为 2 2 9、2 50、2 2 9、2 65、2 2 5nm,表明吸收光谱严重重叠。在相应的 λmax处 ,其表观摩尔吸光系数分别为 5.61× 1 0 3、7.48× 1 0 3、6.1 5× 1 0 3、7.89× 1 0 3以及 4.35× 1 0 3L· mol-1· cm-1。用偏最小二乘法和迭代目标转换因子法等化学计量学法辅助紫外分光光度法 ,不经分离 ,同时测定了模拟样和小儿苯巴比妥片剂试样中的上述四组分。虽然体系的加和性较差 ,但由于两算法抗交互作用和抗随机误差的能力较强 ,故仍取得了满意的结果。

The absorption maxima of aspirin,phenacetin,caffeine and phenobarbital in 0.5 g/L tartaric acid alcoholic solution lie at 229,250,229 and 265,and 225 nm,respectively ,indicating strong overlapping absorption spectra.At the corresponding λ max ,their apparent molar absorptivities are 5.61×10 3, 7.48×10 3, 6.15×10 3, 7.89×10 3 and 4.35×10 3 L·mol -1 ·cm -1 ,respectively.The above mentioned four components in simulated samples and compound child′s phenobarbital tablet sample have been simultaneously determined using UV spectrophotometry assisted by some chemometrics methods such as partial least squares algorithm and iterative target transform factor analysis algorithm without separation .Although the additivity for the systems was poor,satisfactory results were still achieved because of strong anti interaction and anti-random error abilities of two algorithms.This method is convenient and fast,has good accuracy and precision,and can be applied to monitor the quality of compound drugs.