以酸性铬蓝K作为氢供体的酶催化光度法测定过氧化氢

Catalytic Photometry with Enzyme for Determination of Hydrogen Peroxide by Using Acid Chrome Blue K as Hydrogen Donor

研究了以酸性铬蓝 K作为氢供体底物的过氧化氢酶 -过氧化氢催化反应体系 ,拟定了测定痕量过氧化氢的新的酶催化光度法。测得该体系的最大反应速率 Vmax值为 6.2 5× 1 0 -3 mol· L-1·S-1,米氏常数 Km值为 2 .78× 1 0 -5mol/L。测定过氧化氢的线性范围为 0 .0 3～ 0 .6mg/L。检出限为 4.6× 1 0 -4 mol/L。

A study on the acid chrome blue K as a hydrogen donor substrate of catalase hydrogen peroxide system is reported. A new catalytic spectrophotometric method for the determination of trace amount of hydrogen peroxide was developed.The maximum velocity V m was 6.25×10 -3 mol·L -1 ·S -1 for the catalytic reaction,and michaelis constant K m is 2.78×10 -5 mol/L. The linear range is 0.03～0.6 mg/L for hydrogen poroxide,and detection limit was 4.6×10 -4 mg/L. The method has been used for the determination of hydrogen peroxide in rain water.