菊花主要活性成分含量及其抗氧化活性测定

Bioactive Component Content and Antioxidant Activity of Chrysanthemum Flower (Chrysanthemum morifolium Ramat) Extract

目的:建立测定菊花中主要活性成分含量的方法并对其抗氧化活性进行测定。方法:利用高效液相色谱法(HPLC)测定不同菊花品种提取物中绿原酸、木犀草苷、槲皮素、木犀草素和芹菜素的含量;测定菊花总黄酮的含量及其抗氧化活性,考察两者的相关性。结果:不同品种菊花中的生物活性成分含量各异,贡菊中绿原酸含量最高,为9.8mg/g;杭白菊中的木犀草苷和槲皮素含量最高,分别为0.94、2.76mg/g;亳菊中木犀草素含量最高,为2.48mg/g;滁菊中芹菜素含量最高,为0.20mg/g。贡菊和杭白菊总黄酮提取率分别为5.07%、2.65%。贡菊、杭白菊和市场购得的菊花提取物相比,贡菊的T-AOC能力、清除.OH能力和DPPH自由基清除率能力均为最强,分别为(51.22±1.66)、(57.38±1.39)U/mg和(89.68±0.23)%,菊花总黄酮含量与各抗氧化指标有一定的相关性,相关系数(R2)分别为0.993(P<0.05)、0.968(P<0.05)和0.977(P<0.05)。结论:不同品种菊花中的活性成分含量各异,贡菊的抗氧化活性较强且与总黄酮的含量有一定的相关性。

Objective： To establish a method for determining the contents of major bioactive components in chrysanthemum flower and evaluate the antioxidant activity of chrysanthemum flower extract. Methods： HPLC was used to determine the contents of chlorogenic acid, luteoloside, quercetin, luteolin and apigenin in chrysanthemum flower extract. Total flavonoids and antioxidant activity of chrysanthemum flower extract were determined to explore the correlation between both indexes. Results： Bioactive components in different chrysanthemum cultivars were different. The highest level of chlorogenic acid was observed in Gong Ju, which was 9.8 mg/g. The highest levels of luteoloside and quercetin were detected in Hangbai Ju, which were 0.94 mg/g and 2.76 mg/g, respectively. The highest level of luteolin was determined in Bo Ju, which was 2.48 mg/g. The highest level of apigenin was observed in Chu Ju, which was 0.20 mg/g. The total flavonoids in Gongju and Hangbai Ju were 5.07% and 2.65%, respectively. Compared with Hangbai Ju flower extract and commercial chrysanthemum flower extract, Gongju flower extract had higher T-AOC and scavenging activity against hydroxyl and DPPH free radicals, which were （51.22 ± 1.66） U/mg, （57.38 ± 1.39） U/mg and （89.68 ± 0.23）%, respectively. An excellent correlation between antioxidant properties and total flavonoids content was observed （R2 = 0.993, P 〈 0.05 for T-AOC; R2 = 0.968, P 〈 0.05 for hydroxyl free radical scavenging activity; R2 = 0.977, P 〈 0.05 for DPPH free radical scavenging activity）. Conclusion： Chrysanthemum morifolium Ramat flowers from different cultivars have different bioactive components. Gongju has the strongest antioxidant activity, which is related to total flavonoids.