1株蒜薹采后病原真菌的鉴定、rDNA ITS序列及碳源代谢指纹图谱分析

Identification, rDNA ITS Analysis and Carbon Metabolic Fingerprinting of a Pathogenic Fungus Isolated from Postharvest Garlic Sprout

为明确蒜薹贮藏期致病菌种类,增强采后病害防治针对性,从自然发病的苍山草薹上分离到1株丝状致病真菌,经形态学特征观察及rDNA ITS序列分析确定为茄匍柄霉(Stemphylium solani),分析其对95种碳源代谢指纹图谱发现该菌对碳源适应性较强,最适碳源有D-果糖、蔗糖、麦芽糖、α-D-葡萄糖、D-半乳糖等75种,可利用碳源有5种:D-葡萄糖胺、D-塔格糖、γ-羟丁酸、D-苹果酸和尿苷,不可利用碳源有N-乙酰-β-D-甘露糖胺、N-乙酰-D-半乳糖胺、L-海藻糖、i-赤藓糖醇、葡糖醛酰胺等15种。

A pathogenic fungus was isolated from infected garlic sprout during postharvest storage. Morphological observation and rDNA ITS analysis indicated that the isolated strain was Stemphylium solani. Metabolic fingerprinting investigation of 95 carbon sources suggested that the carbon metabolic fingerprinting of the strain was composed of 75 optimal carbon sources, including D-fructose, sucrose, maltose, a-D-glucose, D-galactose, and 5 available carbon sources （D-glucosamine, D-tagatose, hydroxybutyric acid, D-malic acid and uridine） as well as 15 unavailable carbon sources such as N-acetyl-D-galactosamine, N-acetyl-fl-D-mannosamine and i-erythritol.