摘要
以荔枝壳原花青素低聚体(LPOPC)为研究对象,对SD大鼠连续给药1周,给药剂量150mg/(kg.d)。给药期间,收集动物灌胃后第1、2、3、5、7天的排泄物,并分析动物给药前后尿液和粪便性质的改变与差异。结果表明:给药后大鼠尿液的总抗氧化能力显著升高(P<0.01),且与尿液中总酚类物质的含量呈现相关性。在电子鼻分析仪中,给药组大鼠的粪便气味响应值明显低于空白组;HS-SPME-GC-MS分析证实,原花青素灌胃后粪便中挥发性物质苯甲醛、4-甲基苯酚等的强度升高,而产生腐败气味的吲哚、4-乙基苯酚等物质的含量则降低。对粪便中原花青素的代谢产物进行鉴定,发现了单体(-)-表儿茶素和m-香豆酸的存在。
An increasing amount of attention is being paid to litchi pericarp procyanidins due to its strong antioxidant activity in vitro and in vivo. Litchi pericarp oligomeric procyanidins （LPOPC） were investigated in this study. SD rats were administered with LPOPC at the dose of 150 mg/（kg.d） for 1 week. The urine and feces were collected on the 1st, 2nd, 3rd, 5th and 7th day during LPOPC administration to compare excreta characteristics from the control group. LPOPC administration resulted in a significant elevation in the T-AOC of rat urine, which was linked to total phenol content. However, the fecal odor of rats revealed a decreasing trend after the administration of LPOPC as illustrated by electronic nose analysis, which was due to the reduction in putrefactive products such as indole and 4-ethyl phenol in feces and an increasing amount of volatile compounds such as benzaldehyde and 4-methyl phenol as identified by HS-SPME-GC-MS. In addition, LC-MS analysis of procyanidin metabolites in feces suggested that （-）-epicatechin and m-coumaric acid were the maj or components.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2013年第15期267-271,共5页
Food Science
基金
"十二五"国家科技支撑计划项目(2012BAD31B03)
