党参多糖抗氧化作用及其对果蝇寿命的影响

Antioxidant Activity of Polysaccharide from Codonopsis pilosula in Aging Model Mice and Its Effect on Senescence Resistance of Fruit Flies

目的:研究党参多糖(CPPS)抗氧化及延缓衰老的作用。方法:选用D-半乳糖(D-gal)诱导建立衰老模型,CPPS分别按高剂量(250mg/(kg.d))、中剂量(150mg/(kg.d))、低剂量(50mg/(kg.d))连续灌胃50d,观察CPPS对小鼠脑、肝脏组织超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、丙二醛(MDA)的影响;用CPPS高剂量(0.08%)、中剂量(0.016%)、低剂量(0.0032%)培养果蝇,进行果蝇生存实验。结果 CPPS可以显著提高SOD、GSH-Px的活性(P<0.05或P<0.01),降低MDA含量(P<0.05)。高、中剂量CPPS都显著提高果蝇平均寿命和最高寿命(P<0.05)。结论 :CPPS具有一定抗氧化延缓衰老的作用。

Objective： To explore the effect of Codonopsis pilosula polysaccharide （CPPS） on oxidation and senescence resistance. Methods： Aging mouse models were induced by injection of D-galactose. CPPS at high, middle and low doses were intragasWically administered to aging model mice. Malondialdehyde （MDA） content, and glutathione peroxidase （GSH-Px） and superoxide dismutase （SOD） activities in brain and liver homogenates were measured. According to different polysaccharide doses, the survival of fruit flies was also investigated. Results： CPPS could improve the activities of SOD and GSH-Px （P 〈 0.05, P 〈 0.01） and decrease the content of MDA in brain and liver homogenates （P 〈 0.05）. The average life span and maximum life span in the high and middle dose groups were significantly higher than those in the control group （P 〈 0.05）. Conclusion： CPPS has obvious roles in anti-oxidation and senescence resistance.