摘要
目的:利用动物模型和体外模拟胃液消化实验对常见食品过敏原(11S大豆球蛋白、卵清白蛋白(OVA))和非食品过敏原马铃薯酸性磷酸酶(PAP)的致敏性进行一致性分析。方法:采用经口灌胃的方式用3种食物蛋白致敏BALB/c雌性小鼠,ELISA法测定血清中特异性IgE水平和血浆中组胺水平;对3种食物蛋白进行模拟胃液消化,研究蛋白在体外模拟胃液中的消化稳定性。结果:动物模型实验结果显示11S大豆球蛋白的致敏性最强,其次是OVA,PAP的致敏性很低;体外模拟胃液消化结果显示11S大豆球蛋白对胃蛋白酶的抗消化性高于OVA,PAP不具有抗消化性。结论:动物模型和模拟胃液消化的方法在评价食品中过敏原致敏性的结果基本一致。
Purpose： To analyze the potential allergenicity of common food allergens such as 11S globulin and ovalbumin （OVA） and non-allergen such as potato acid phosphatase （PAP） using two methods： animal model in vivo and simulated gastric digestion in vitro. Methods： Female BALB/c mice were orally administrated with three food proteins. Protein- specific IgE antibodies in serum and plasma histamine levels were detected by ELISA. Additionally, the food proteins were digested in simulated gastric fluid in vitro to explore the digestive stability. Results： The results of animal model showed that 11S globulin had the strongest allergenicity, followed by OVA and PAP. The results of protein digestion by pepsin showed that the anti-digestion capability of 11S globulin was higher than that of OVA, and PAP was not a digestion-stable protein. Conclusion： For the most of the food allergens, the results of animal model and simulated gastric digestion in vitro are consistent.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2013年第15期313-317,共5页
Food Science
关键词
食品过敏
过敏原
动物模型
模拟胃液消化
food allergen
allergenicity
animal model
simulated gastric digestion
