非小细胞肺癌细胞周期调节蛋白A1及乳腺癌易感基因1基因甲基化研究

Gene methylation of Cyclin A1 and breast vancer dusceptibility gene 1 in non-smallcell lung cancer

目的探讨细胞周期调节蛋白A1（细胞周期素A1，CCNA1）及乳腺癌易感基因1（BRCA1）甲基化在非小细胞肺癌（NSCLC）患者中的发生频率。方法采用甲基化特异性聚合酶链反应（MSP）方法检测50例临床上经病理确诊为非小细胞肺癌患者（实验组）和20例良性肺部疾病患者（对照组）CCNA1及BRCA1甲基化的状态，以建立广西非小细胞肺癌相关基因的甲基化谱式。结果在50例非小细胞肺癌患者CCNA1及BRCA1甲基化率分别为70．0％（35／50）和84．0％（42／50）。对照组中20例良性肺部疾病患者这2个基因均为去甲基化。结论检测非小细胞肺癌患者CCNA1及BRCA1基因DNA甲基化状态有助于诊断非小细胞肺癌。

Objective To explore the frequency of Cyclin A1 （ CCNA1 ） and breast vancer dus- ceptibility gene 1 （ BRCA1 ） gene methylation in patients with non-small cell lung cancer （NSCLC）. Meth- ods The methylation specific polymerase chain reaction （MSP） was used to measure the gene methylation status of CCNA1 and BRCA1 in 50 patients with clinically pathologically confirmed NSCLC （ experimental group） and 20 patients with benign pulmonary diseases （control group） in order to establish the spectral type of Guangxi NSCLC related genes. Results In 50 cases of NSCLC, CCNA1 and BRCA1 methylation rate was 70. 0% （ 35/50 ） and 84. 0% （ 42/50 ） respectively. In control group, the DNA of these two genes was demethylated. Conclusion The detection of CCNA1 and BRCA1 gene DNA methylation status is beneficial for the diagnosis of Guangxi NSCLC.