盐霉素对乳腺癌转移的抑制作用

Inhibitory effect of salinomycin on invasion of breast cancer ceils

目的观察盐霉素对乳腺癌细胞株MCF-7、MCF-7／ADR、MDA—MB-231的体外增殖和侵袭能力的影响，探讨其对乳腺癌细胞的转移抑制作用。方法用细胞计数试剂盒（CCK-8）法检测细胞增殖，得出半数抑制浓度（IC50）值；用Transwell小室法检测侵袭能力的变化；分别用实时定量聚合酶链反应（Real—timePCR）法和Westernblot法检测ETS1和基质金属蛋白酶（MMP）-1基因和蛋白的表达变化。结果盐霉素抑制乳腺癌细胞的增殖，3种细胞的IC50值分别为（7．47±0．64）、（5．11±0．97）、（4．08±0．85）μmoL／L；经不同浓度的盐霉素处理后，3种细胞侵袭能力逐渐下降，分别为对照组的65．9％／41．9％、55．8％／37．8％、48．5％／24．6％；MCF-7／ADR和MDA—MB-231的ETS1和MMP-1表达水平随着盐霉素浓度升高而下降。结论盐霉素可以抑制乳腺癌细胞的增殖，降低其侵袭能力。

Objective To study the effects of salinomycin on the proliferation and invasion of breast cancer cells and the mechanism. Methods The human breast cancer cells MCF-7, MCF-7/ADR and MDA-MB-231, were treated with salinomycin. Cell viability was determined by cell counting kit-8. The invasive ability of tumor cells was evaluated by Transwell chamber assay. The mRNA and protein levels of ETS1 and matrix metalloproteinase （MMP） -1 were detected by using real-time quantitative polymerase chain reaction （Real-time PCR） and Western blotting respectively. Results Salinomycin significantly in- hibited the proliferation of breast cancer cells with the IC50 value being （7.47 ±0. 64） for MCF-7, （5. 11 ±0. 97） for MCF-7/ADR, and （4. 08 ±0. 85） μmol/L for MDA-MB-231, respectively. The invasion capac- ity of breast cancer cells were significantly reduced by salinomycin （65.9%/41.9% for MCR-7, 55.8%/ 37.8% for MCR-7/ADR, and 48. 5%/24. 6% for MDA-MB-231, respectivel）. Simustaneously, the ex- pression levels of ETS1 and MMP-1 were downregulated by salinomycin. Conclusion Salinomyein can in- hibit the proliferation of breast cancer cells, and reduce the invasion capacity.