摘要
实验建立了分子印迹固相萃取-高效液相色谱法检测饲料中联苯三唑醇。采用本体聚合法自制联苯三唑醇分子印迹聚合物(MIP),并以此聚合物为固相萃取剂,对饲料样品中联苯三唑醇进行了分离富集。利用高效液相色谱法测定其在饲料中的残留情况。结果表明:该方法线性范围为1～200μg/mL,检测限为0.5μg/mL,平均回收率为76.4%～89.0%。
Moleculary imprinted solid phase extraction-high performance liguid chromatography method was estab- lished for determination of bitertanol in feed.Bitertano] molecularly imprinted polymer （MIP）was prepared by mass poly- merization,and was used as solid phase extraction agent to separate and enrich bitertanol in feed.Bitertanol residua in feed was determined by high performance liquid chromatography （HPLC）.The results showed that：linear range was 1 - 200 μg/ mL, and the detection limit was 0.5 μg/mL.The average recovery was between 76.4% and 89.0%.
出处
《中国饲料》
北大核心
2013年第15期33-34,43,共3页
China Feed
基金
河北省科技支撑计划项目(10276902D)
石家庄市科技支撑计划项目(12149472A)
关键词
联苯三唑醇
分子印迹聚合物
固相萃取
高效液相色谱法
bitertanol
molecularly imprinted polymer
solid phase extraction
high performance liquid chromatography
