豚鼠双侧颈迷走神经切断对肺功能的影响

Effect of Bilateral Cervical Vagotomy on Lung Function in Guinea Pig

目的 :观察豚鼠双侧颈迷走神经切断急性肺损伤对肺功能的影响。方法 :将 16只动物随机分为两组 ,正常对照组 (Ⅰ组 ,n =8)为无迷走神经切断纯氧通气观察 2小时 ,迷走神经切断组 (Ⅱ组 ,n =8)为迷走神经切断纯氧通气PaO2 <2 0kPa后观察 2小时期间血气分析、肺顺应性、血管外肺水及组织学变化。结果 :迷走神经切断后 75 1± 18 9分钟可出现明显的PaO2 下降、PaCO2 升高、CL 降低 (P <0 0 1) ,MAP和HR基本稳定 ,随肺损伤进展出现BP渐降 (P>0 0 5 )、HR渐缓 (P <0 0 5 ) ;血管外肺水显著增加 ,超氧化物歧化酶 (SOD)活性降低 (P <0 0 1)。结论 :豚鼠双侧颈迷走神经切断可产生通透性肺水肿 。

Objective:Toevaluatetheeffectsofbilateralcervicalvagotomyofadultguineapigonlungfunction .Methods:16 guineapigswererandomlydividedintotwogroups.Nonvagotomizedguineapigsweremechanicallyventilatedwith 10 0 %oxygenexceptthatthenerveswerenotcutingroupⅠ (n =8) .Andguineapigsweremechanicallyventilatedwith 10 0 %oxygenuntillthePaO2 waslowerthan 2 0kPafollowingbilateralcervicalvagotomyingroupⅡ (n =8) .Gasexchange,lungcomplianceandextravascularlungwaterweremeasuredwithin 2hours.Results:AreductionofPaO2 ,pulmonarycompliance andanincreaseofPaCO2 about 75 1± 18 9minweresignificant(P <0 0 1) .Themeanarterialbloodpressure(MAP)and heartrate(HR)didnotchangedfollowingvagotomy ,butlatechangesinMAP(P >0 0 5 )andHR(P <0 0 5 )occuredafter respiratoryfailure .Theextravascularlungwaterincreased ,theactivityofsuperoxidedismutase (SOD)decreased(P <0 0 1) . Conclusion :Vagotomizedguineapigresultsinproteinaceouspermeabilitypulmonaryedemaassociatedwithgasexchangefail ure .