丝瓜瓤固定化脱色菌Enterobactor sp.S8对活性黑5的脱色

Decolorization of Reactive Black 5 by strain Enterobactor sp.S8 immobilized on Luffa cylindrica sponge

利用植物载体丝瓜瓤对肠杆菌(Enterobactor sp.S8)进行固定,并对活性黑5进行脱色研究。探讨了固定化菌体的菌龄、菌量、pH、温度对染料脱色的影响。研究了该固定化菌体的重复利用和动力学实验。结果表明,最佳脱色条件为:菌龄3 d、接菌量2%、温度30℃、pH 6.0。该固定化菌体对不同初始浓度活性黑5的脱色符合二级反应动力学方程。经9次重复利用后的该固定化菌体,其脱色率仍达76.8%。在优化实验条件下,根据降解3 d前后的紫外-可见光谱图分析可知,活性黑5并非完全被降解为CO2和H2O,而是生成一些小分子有机中间体。

The decolorization of Reactive Black 5 by Enterobactor sp.S8 immobilized on Luffa cylindrica sponge was studied.The effects of bacteria age,bacteria amount,pH and temperature on decolorization were discussed.The reusability and kinetics of the immobilized bacteria were also studied.The results showed that the optimum conditions for decolorization by immobilized bacteria were 3-day culture,inoculation of 2%,temperature of 30℃,and pH 6.0.The decolorizaton of immobilized bacteria on Reactive Black 5 under various initial concentrations conformed to the second-order reaction mode.After nine times reused,the decolorization rate of the immobilized bacteria could still reach 76.8%.According to the UV-Vis spectrum before and after 3 d degradation under the optimum conditions,Reactive Black 5 was not completely mineralized to CO2 and H2O.Instead,some organic intermediates were produced.