新型尿苷肽类化合物Sansanmycin P的提取分离、结构鉴定及活性研究

Isolation, structural determination and biological activity of novel uridyl peptide compound Sansanmycin P

目的从放线菌Streptomyces sp SS发酵液中分离新型活性尿苷肽类似物。方法发酵液经D4006大孔吸附树脂、DEAE-葡聚糖凝胶及制备液相分离纯化,获得单一化合物;根据UV、MS、MS/MS、1D和2D NMR确定化合物结构,并进行抗菌活性研究。结果分离纯化得到1个N-末端含有1-甲基-6-羟基四氢异喹啉的尿苷肽类化合物Sansanmycin P。初步体外抗菌活性,抗铜绿假单胞菌MIC>32μg/ml,抗结核杆菌MIC>32μg/ml。结论 Sansanmycin P为全新结构Sansanmycins类似物,具有一定抗结核杆菌和铜绿假单胞菌活性。

Objective To isolate new uridyl peptide components from the fermentation broth of Streptomyces sp. SS. Methods The new component was isolated and purified by various chromatography methods, such as macroporous adsorption resins, ion exchange resin and reversed-phase HPLC. Its structure was elucidated by spectroscopic analyses, such as UV, MS, MS/MS, 1D and 2D NMR.Results A novel uridyl peptide antibiotic, Sansanmycin P, with an N-terminal containing 1-methyl-6-hydroxy-1,2,3,4-tetrahydro-3-isoquinoline carboxylic acid was obtained. Antibacterial activity in vitro, resistance of Pseudomonas aeruginosa MIC 〉 32 μg/ml, Mycobacterium tuberculosis MIC 〉 32 μg/ml. Conclusion Sansanmycin P is a novel Sansanmycin analogue with certain antimicrobial activities against Mycobacterium tuberculosis and Pseudomonas aeruginosa.