妊高征患者母血脐血染色体稳定性的研究及其临床意义

Study on the chromosome stability in maternal and umbilical blood of PIH and its clinical significance.

目的 观察妊娠高血压综合征 (PIH)孕妇外周血及其脐血姐妹染色单体互换 (SCE)频率 ,探讨PIH的病因以提高PIH的检测水平。方法 采用姐妹染色单体互换 (SCE)法和放免法分别对 2 1例PIH病人和 15例正常孕妇 (对照组 )的外周血及其所分娩新生儿脐血SCE频率的测定。结果 PIH组外周血和脐血SCE频率分别为 (6 11± 1 2 9)次 ,(5 98± 1 38)次 ;对照组分别为 (3 45± 0 71)次 ,(3 16± 0 5 7)次。其两组比较 ,差异有极显著性 (P <0 0 1)。PIH组外周血和脐血SCE频率皆异常。结论 PIH与遗传DNA物质损伤有关 ,SCE频率可作为一项诊断、预测PIH的重要指标。

Objective To study sister chromatid exchange(SCE) frequency in maternal peripheral lymphocytes and umbilical blood of pregnant women.It is objective to study causes and best assay about PIH.Methods The frequency of SCE in peripheral blood lymphocytes and radioimmunoassay of 21 PIH mothers and 15 normal ones as well as the umbilical blood were investigated by both SCE method and radioimmunoassay.Results The mean frequency of SCE in maternal and umbilical blood with normal pregnancy were (3 45±0 71) and (3 16±0 57),respectively,while they were (6 11±1 29) and (5 98±1 38) in PIH group, respectively.The difference of SCE levels between the two groups was statistically significant(P<0 01).Conclusion One of the causes of PIH may be related to heredity injury.Thus,SCE will be a new index to diagnose and predict PIH.