猪外周血淋巴细胞与心肌细胞βAR的相关性研究

βAR relating study of porcine circulating lymphocytes and myocardial tissue

目的 对猪外周血淋巴细胞膜上 βAR与心肌细胞膜上 βAR的定量检测 ,研究两者之间的相关性。 方法 对17例 7月龄的猪 ,取外周血及心肌组织 ,用3 H -DNA放射配基法测定其 βAR密度。 结果 猪外周血淋巴细胞膜上 βAR与心肌细胞膜上βAR呈显著相关 (r=0 0 95 6 ,P <0 0 0 1)。 结论 对猪外周血淋巴细胞膜上 βAR的检测 ,可间接反映其心肌细胞膜上 βAR的变化。因而可应用于实验性急救药物研究。

Objective To study βAR relation between porcine myocardial tissue and circulating lymphocyte beta-adrenoceptor density.Method βAR density were measured in the myocardial tissue and circulating lymphocyte of 17 pigs.βAR density were determined by 3H-dihydroalpneold as ligand.Result βAR density were important relation between myocardial tissue and circulating lymphocyte( r =0 9056, P <0 001).Conclusion βAR density of circulating lymphocyte can reflect the alterations of myocardial tissue.Therefore,makes it possible to study drug-induced βAR changes. [