MicroRNA-31在食管鳞状细胞癌中的表达及其与预后的关系

The expression of microRNA-31 in esophageal squamous cell carcinoma and its prognostic value

背景与目的:研究发现,许多微小RNA(microRNAs,miRNAs)与恶性肿瘤密切相关,其中miR-31(microRNA-31)在许多肿瘤中呈异常改变。中国是食管鳞状上皮细胞癌(esophageal squamous cell carcinoma,ESCC)最高发的地区之一。本研究旨在调查miR-31在ESCC中的表达水平与临床病理学因素以及预后的关系。方法:采用实时反转录聚合酶链反应(reverse transcription-polymerase chain reaction,RTPCR)技术检测食管癌细胞株KYSE410、EC1和EC9706,以及81例ESCC组织和癌旁正常组织中miR-31的表达水平。结果与临床资料和随访结果结合作统计分析。结果:在3株ESCC细胞株以及75.31%的ESCC组织中,miR-31表达上调。并且miR-31的高表达与更严重的淋巴结转移(P=0.043)、更深的浸润深度(P=0.002)以及更晚期的病理分期有关(P=0.027),而与其他临床病理学因素无关(P>0.05)。此外,miR-31的高表达与较差的无进展生存期(progression-free survival,PFS)有关(Kaplan-Meier分析P=0.014,多变量Cox分析P=0.021)。结论:miR-31可能是ESCC一项新的诊断和预后评价指标。

Background and purpose： It was reported that many microRNAs （miRNAs） have close relation with carcinomas, miR-31 （microRNA-31） shows abnormal change in numerous cancers. China is one of the most high-risk areas of esophageal squamous cell carcinoma （ESCC）. The aim of the present study was to investigate the expression of miR-31 in ESCC, and analyze the relationship of its expression with clinicopathological features and prognosis. Methods： The expression ofmiR-31 in KYSE410, EC1 and EC9706 cell lines, as well as 81 cases of ESCC tissues and adjacent normal esophageal tissues were detected by real-time reverse transcription-polymerase chain reaction （RT-PCR）. The result was combined with clinical and follow-up data and statistical analysis was conducted. Results： MiR-31 was up-expression in 3 cell lines and 75.31% of the ESCC tissues, miR-31 up-expression was positively related to severer lymph node metastasis （P=0.043）, deeper invasion of tumors （P=0.002） and advanced pathological stage （P=0.027）. There was no relationship of miR-31 with other clinicopathological features （P〉0.05）. Furthermore, high expression of miR-31 was associated with poor progression-free survival （PFS） in 81 ESCC patients by Kaplan-Meier analysis （P=0.014） and by multivariate Cox analysis （P=0.021）. Conclusion： Our results identified miR-31 may be a new diagnostic criteria and prognostic biomarker for ESCC.