摘要
目的:观察益肾胶囊对糖尿病肾病大鼠肾组织SOCS-3、Ⅰ、Ⅳ型胶原表达的影响,探讨益肾胶囊延缓糖尿病肾病肾纤维化的机制。方法:36只健康雄性Wistar大鼠,通过尾静脉单次注射链脲佐菌素(60 mg/kg),制造糖尿病大鼠模型,后随机分为DN模型组、益肾胶囊组、氯沙坦钾组,各12只,益肾胶囊组每只灌胃益肾胶囊(625 mg.kg-1.d-1),氯沙坦组每只灌胃氯沙坦钾片(30 mg.kg-1.d-1),另取12只健康大鼠作为正常对照组。正常对照组及模型组每日给予等量蒸馏水。12周后测定各组大鼠体重、24 h尿蛋白定量、血肌酐、尿素氮;采用HE、Masson和PAS染色观察肾组织病理变化,采用免疫组化法检测肾组织中SOCS-3、Ⅰ、Ⅳ型胶原表达水平。结果:实验12周末,与正常组比较,模型组大鼠病理改变较明显,24 h尿蛋白定量、血肌酐、尿素氮明显上升(P<0.05),肾组织中SOCS-3、Ⅰ、Ⅳ型胶原表达明显上调。与模型组比较,治疗组大鼠病理改变减轻,24 h尿蛋白定量、血肌酐、尿素氮水平明显降低(P<0.05);肾组织中SOCS-3表达显著上调,Ⅰ、Ⅳ型胶原表达显著抑制(P<0.05)。结论:益肾胶囊可能通过上调SOCS-3表达,抑制Ⅰ、Ⅳ型胶原的表达,从而减轻了DN大鼠肾小球硬化和肾小管间质纤维化的程度,延缓糖尿病肾病的进展。
Objective：To observe the effect of Yishen capsule on SOCS -3 ,Collagen type Ⅰ and Ⅳ in Diabetic nephropathy rats and to investigate the mechanism of Yishen Capsule to delay diabetic nephropathy fibrosis. Methods： The rat models of diabetic nephropathy were established by intraperitoneal injection of STZ （ Streptozotocin 60 mg/kg）. Then the rats were randomly divided into three groups： DN group（only distilled water by gavage）, Y group （Yishen capsule 625 mg.kg-1. d-1 by gavage）, L group （Losar- tan 30 mg . kg-1. d-1 by gavage） . The additional set of healthy rats were given no STZ were used as control group （N group）. Each group consisted of 12 rats. By 12 weeks, the body weight, 24 h urinary albumin excretion, serum creatinine （Scr） and blood u- rea nitrogen （BUN） level were measured in each rat. Then, renal tissue samples were observed under optical microscope. Expression of SOCS - 3 ,Collagen type Ⅰ , Ⅳ in ream tissue samples were detected by immunohistochemistry. Results： Compared with N group, DN group had significant elevated 24 h urinary albumin, Scr and BUN （ P 〈 0.05 ）, the pathological changes in the renal tissue sam- ples in DN group were also obvious. Compared with DN group, the treated groups had significant lower 24h urinary albumin, Scr and BUN （P 〈 0.05 ）. The pathological changes in the renal tissue samples were much relieved in the treated group. The expression level of SOCS3 in treated group was significant higher than that in DN group, but the expression level of Collagen type Ⅰ , Ⅳ were lower than that in DN group （ P 〈 0.05 ）. Conclusion： Yishen capsule may through the up - regulation of SOCS3 expression to inhibit the ex- pression of Collagen type Ⅰ , Ⅳ, thus reduce the DN rat glomerular sclerosis and renal tubule interstitial fibrosis degree, then delay the progress of diabetic nephropathy.
出处
《中国中西医结合肾病杂志》
2013年第7期573-575,共3页
Chinese Journal of Integrated Traditional and Western Nephrology
基金
国际科技合作项目(No.2011081066)
国家自然基金资助项目(No.81173364)
