积雪草颗粒对UUO大鼠肾组织CTGF、α-SMA、HGF、MCP-1表达的影响

Effects of Centella Asiatica Particles on Renal Tissue in UUO Rats CTGF,α - SMA,HGF,MCP - 1 Expression

目的:探讨积雪草对单侧输尿管梗阻(UUO)大鼠肾组织结缔组织生长因子(CTGF)、α-肌动蛋白(α-SMA)、肝细胞生长因子(HGF)、单核细胞趋化蛋白(MCP-1)-1表达的影响,为临床上应用积雪草延缓肾衰竭提供依据。方法:50只雄性SD大鼠随机分为假手术组、模型组、积雪草大剂量组、积雪草小剂量组、对照(福辛普利)组。每组10只,除假手术组外均成功建立UUO大鼠模型,术后21 d腹主动脉采血,检测肝、肾功能的变化;免疫组化检测CTGF、α-SMA、HGF、MCP-1的表达部位及含量。结果:模型组CTGF、α-SMA、MCP-1的表达高于假手术组(P<0.01),药物干预组指标低于模型组(P<0.01);药物干预组HGF含量显著高于模型组(P<0.01);其中积雪草大剂量组的表达高于小剂量组(P<0.01),但与福辛普利组相似(P>0.05)。药物干预组BUN水平下降,Scr、ALT、AST变化不明显。结论:积雪草可通过上调UUO大鼠肾组织HGF的表达,抑制UUO大鼠肾组织CTGF、α-SMA、MCP-1表达而实现其抗肾小管间质纤维化(TIF)的作用,大剂量组与福辛普利组作用相似,且没有明显的肝肾毒性。

Objective ：To investigate the effect of Centella asiatica on unilateral ureteral obstruction （UUO） connective tis- sue of CTGF, α - SMA, HGF, MCP - 1 expression, thus providing scientific evidence For the clinical application of Centella asiatiea delaying renal failure. Methods ：50 male SD rats were randomly divided into sham operation group, model group, low -dose Centella asiatica group,middle- dose group,high -dose group,and control group , 10 rats in each group. UUO rat models were successfully established in all groups with the exception of sham group. 21 days after operation ,abdominal aortic blood collection for the examina- tion of liver and kidney functions and immunohistoehemical staining for the deteetion of localization and expression tendency of CTGF α -SMA, HGF and MCP- 1 protein in renal tissues. Results：The expression of CTGF,α-SMA, MCP- 1 of model group, higher than those in sham operation group （P 〈0.01 ）, drug intervention group lower than that of model group （P 〈0.01 ） ; drug interven- tion in HGF group was significantly higher than that in the model group （ P 〈 0.01 ） ; the expression of Centella asiatica in high dose group was higher than that of small dose group （P 〈 0.01 ）, but similar with Fosinopril group （ P 〉 0.05 ）. Drug intervention group decreased levels of BUN, Ser, ALT, AST did not change significantly. Conclusion：By up -regulating the expression of HGF in renal tissue of UUOrats ,and inhibiting CTGF,α- SMA, MCP - 1 expression of renal tissue in UUO rats to reduced the development of tu- bulointerstitial fibrosis, the Centella asiatica high dose group and Western medicine control group efficacy and no significant liver tox- 1city