菌草灵芝菌糟的饲用安全性

Safety of spent substrate after Ganoderma lucidum cultivation with Jucao as feed

通过小鼠急性毒性试验、30 d喂养试验、黄曲霉毒素B1的测定和重金属测定,研究菌草灵芝菌糟的饲用安全性.结果表明:小鼠经口的急性毒性MTD>20 g.kg-1,对小鼠无不良影响;30 d喂养试验期间,各组小鼠的生长不受影响,且添加5%、15%灵芝菌糟组小鼠日增重分别比对照组提高21.62%(P>0.05)、27.03%(P<0.05).剖检未见组织器官病变,对血常规检测指标基本无影响(P>0.05);黄曲霉毒素B1、重金属含量均在饲料卫生标准限定范围内.

The mice acute toxicity test, 30 day feeding test, determination of aflatoxin B1 , determination of heavy metals were con- ducted to evaluate feed safety of spent substmte after Ganoderma lucidum cultivation with Jucao. The results showed that the oral a- cute toxicity （ MTD 〉 20 g .kg-1 ） had no harmful effect on mice. During 30 day feeding test, mice growth was not affected. The daily weight gains of the groups added with 5% and 15% of spent substrate after Ganoderma luc/dum cultivation with Jucao were 21.62% and 27.03% higher than those of the control group, respectively. Necropsy showed no organ lesions, and no effect on blood routine indexes （P 〉0.05） was found. Aflatoxin B, and heavy metal content was within the limits of the feed sanitation standard.